Dictyostelium discoideum protocols / edited by Ludwig Eichinger, Francisco Rivero
(Methods in molecular biology ; 346)
データ種別 | 電子ブック |
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著者標目 | Eichinger, Ludwig Rivero-Crespo, Francisco NetLibrary, Inc |
出版者 | Totowa, N.J. : Humana Press |
出版年 | c2006 |
書誌詳細を非表示
巻次 | electronic bk. ; ISBN:9781597451444 electronic bk. ; ISBN:1597451444 |
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大きさ | xv, 564 p. : ill. ; 24 cm |
本文言語 | 英語 |
内容注記 | Pt. I. The organism, community resources, and genome-wide studies. The secret lives of dictyostelium / Richard H. Kessin the genome of dictyostelium discoideum / Adam Kuspa and William F. Loomis The cDNA sequencing project / Hideko Urushihara, Takahiro Morio, and Yoshimasa Tanaka dictyBase and the dicty stock center / Petra Fey ... [et al.] Analysis of gene expression using cDNA microarrays / Marcel Kaul and Ludwig Eichinger Proteomic analysis of dictyostelium discoideum / Udo Roth, Stefan Müller, and Franz-Georg Hanisch Pt. II. Basic methods and molecular genetic techniques. Cultivation, spore production, and mating / Hideko Urushihara Parasexual genetics using axenic cells / Jason King and Robert Insall Slug phototaxis, thermotaxis, and spontaneous turning behavior / Paul R. Fisher and Sarah J. Annesley Purification techniques of subcellular compartments for analytical and preparative purposes / Laurence Aubry and Gérard Klein Generation of multiple knockout mutants using the cre-loxP system / Alan r. Kimmel and Jan Faix Restriction enzyme-mediated integration (REM1) mutagenesis / Adam Kuspa RNA interference and antisense-mediated gene silencing in dictyostelium / Markus Kuhlmann, Blagovesta Popova, and Wolfgang Nellen Pt. III. Imaging and localization methods. Application of fluorescent protein tags as reporters in live-cell imaging studies / Annette Müller-Taubenberger Investigating gene expression: in situ hybridization and reporter genes / Ricardo Escalante and Leandro Sastre Application of 2D and 3D DIAS to motion analysis of live cells in transmission and confocal microscopy imaging / Deborah Wessels, Spencer Kuhl, and David R. Soll Using quantitative fluorescence microscopy and FRET imaging to measure spatiotemporal signaling events in single living cells / Xuehua Xu, Joseph A. Brzostowski, and Tian Jin Visualizing signaling and cell movement during the multicellular stages of dictyostelium development / Dirk Dormann and Cornelis J. Weijer Under-agarose chemotaxis of dictyostelium discoideum / David Woznica and David A. Knecht Optimized fixation and immunofluorescence staining methods for dictyostelium cells / Monica Hagedorn, Eva M. Neuhaus, and Thierry Soldati Cryofixation methods for ultrastructural studies of dictyostelium discoideum / Mark J. Grimson and Richard L. Blanton Pt. IV. Dictyostelium as model organism. Analysis of signal transduction: formation of cAMP, cGMP, and Ins(1,4,5)P, in vivo and in vitro / Peter J.M. Van Haastert Assaying chemotaxis of dictyostelium cells / Michelle C. Mendoza and Richard A. Firtel Characterization of cross-linked actin filament gels and bundles using birefringence and polarized light scattering / Ruth Furukawa and Marcus Fechheimer Quantitative and microscopic methods for studying the endocytic pathway / Francisco Rivero and Markus Maniak Preparation of intact, highly purified phagosomes from dictyostelium / Daniel Gotthardt ... [et al.] Assaying cell-cell adhesion / Salvatore Bozzaro Periodic activation of ERK2 and partial involvement of G protein in ERK2 activation by cAMP in dictyostelium cells / Mineko Maeda An improved method for dictyostelium centrosome isolation / Irene Schulz ... [et al.] Epigenetics in dictyostelium / Markus Kaller, wolfgang Nellen, and Jonathan R. Chubb Dictyostelium discoideum as a model to study host-pathogen interactions / Can Ünal and Michael Steinert Pharmacogenetics: defining the genetic basis of drug action and inositol trisphosphate analysis / Kathryn E. Adley, Melanie Keim, and Robin S.B. Williams How to assess and study cell death in dictyostelium discoideum / Artemis Kosta ... [et al.] |
一般注記 | Includes bibliographical references and index Electronic reproduction. Boulder, Colo. : NetLibrary, 2007. Available via World Wide Web. Access may be limited to NetLibrary affiliated libraries |
件 名 | LCSH:Dictyostelium discoideum -- Laboratory manuals
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MESH:Dictyostelium -- cytology -- Laboratory Manuals 全ての件名で検索 MESH:Dictyostelium -- genetics -- Laboratory Manuals 全ての件名で検索 MESH:Genetic Techniques -- Laboratory Manuals 全ての件名で検索 MESH:Models, Biological -- Laboratory Manuals 全ての件名で検索 MESH:Models, Genetic -- Laboratory Manuals 全ての件名で検索 FREE:Electronic books |
分 類 | DC22:579.5/2 |
書誌ID | OB00000318 |
ISBN | 9781597451444 |
NCID | LB40000325 |