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The condensed protocols from molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell

データ種別 図書
著者標目 *Sambrook, Joseph
Russell, David W.
出版者 Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press
出版年 c2006

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府中・第1閲覧一般書 : hardcover 464.1 10557524
0879697725

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巻次 : hardcover ; ISBN:0879697725
: pbk ; ISBN:0879697717
大きさ v, 800 p. ; 29 cm
本文言語 英語
内容注記 Plasmids and their usefulness in molecular cloning
Bacteriophage λ and its vectors
Working with bacteriophage M13 vectors
Working with high-capacity vectors
Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis
Preparation and analysis of eukaryotic genomic DNA
Extraction, purification, and analysis of mRNA from eukaryotic cells
In vitro amplification of DNA by the polymerase chain reaction
Preparation of radiolabeled DNA and RNA probes
Working with synthetic oligonucleotide probes
Preparation of cDNA libraries and gene identification
DNA sequencing
Mutagenesis
Screening expression libraries
Expression of cloned genes in escherichia coli
Introducing cloned genes into cultured mammalian cells
Analysis of gene expression in mammalian cells
Protein interaction technologies
一般注記 Includes index
件 名 LCSH:Molecular cloning -- Laboratory manuals  全ての件名で検索
MESH:Cloning, Molecular -- Laboratory Manuals  全ての件名で検索
分 類 LCC:QH442.2
NLM:QH 440.5
DC22:572.8
書誌ID 1000180071
ISBN 0879697725
NCID BA77594444

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